Isolation and Identification of Proteinase Producing Bacteria and Study on Hydrolysis of Soybean Residue as Medium for Industrial Fermentation
In order to explore the method of using bean dregs as industrial fermentation raw material.Three strains with hydrolyzed protein were isolated from mold bean residue.The phylogenetic tree was constructed by Gram staining,16S rRNA sequencing and database comparison,and the genetic relationship of strains was analyzed.The contents of soluble protein and soluble sugar in fermented soybean residue were analyzed.The industrial bacteria were cultured with soybean dregs fermentation products,and fermented soybean dregs were used as the main raw materials for amino acid fermentation.The results showed that three strains were closely related to Bacillus toyonensis,Stenotrophomonas sepilia and Chryseobacterium hispalense,respectively.The content of soluble protein was up to 4.32 mg/mL,which was 3 times higher than that of unfermented soybean residue.The maximum value of soluble sugar was 5.64 mg/mL,which was twice the soluble sugar content of unfermented soybean residue.Escherichia coli,Pichia pastoris and Corynebacterium glutamate were cultured with fermented products of soybean residue.OD of Pichia pastoris did not exceed 1.5,while OD values of Escherichia coli and Corynebacterium glutamate were 5.0 and 5.7,respectively.The yield of arginine reached 15.8 g/L,and the highest yield of ornithine reached 27.4 g/L.This study offers a theoretical foundation for using soybean residue as medium for industrial fermentation,and provides a new idea for high-value utilization of soybean residue.
万方数据
维普
