Optimization of Extraction Process of Total Flavonoids from Neosinocalamus affinis and Analysis of Antioxidant Activity
Using the Neosinocalamus affinis leaves,branches,and culms as raw materials,based on ultrasonic extraction method.The active ingredients in Neosinocalamus affinis were extracted using alcohol extraction method and water extraction method respectively.The content of total flavonoids,polyphenols,polysaccharides and their antioxidant capacity in the crude extract were determined.At the same time,the extraction process of total flavonoids in Neosinocalamus affinis leaves was optimized.The results showed that when the extraction time was 65 minutes,the ethanol concentration was 77％,and the solid-liquid ratio was 1∶26 g/mL,the total flavonoid content in the alcohol extract of Neosinocalamus affinis leaves was the highest at 2.61％.Meanwhile,there were significant differences in active ingredients and antioxidant capacity among different parts of Neosinocalamus affinis(P＜0.05);The total flavonoid and polyphenol contents in extract of Neosinocalamus affinis leaves were the highest,at 2.58％and 1.75％,respectively.The highest polysaccharide content was found in the alcohol extract of Neosinocalamus affinis branch,which was 4.30％.The antioxidant capacity of alcohol extracts from various parts of Neosinocalamus affinis was significantly higher than that of water extracts,with a ranking of Neosinocalamus affinis leaves＞branches＞culms.
万方数据
维普
