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酱香型白酒第7轮次晾堂清理方式研究

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为保障酱香型白酒生产,基于高通量测序技术和数理统计分析,系统地研究2022年度酱香型白酒下沙投粮生产前制酒晾堂和大曲微生物菌群结构多样性、差异性.从微生物角度分析开水浇烫、蒸煮过的保窖糟摊烫、撒尾酒、撒曲4个环节对制酒晾堂清理的效果.结果表明,酱香型白酒制酒第7轮次晾堂清理前后样品、生产前样品和大曲样品中共检出细菌20个门、336个属,真菌8个门、138个属.明确了Staphylococcus,Oceanobacillus,Ralstonia,Nesterenkonia,Virgibacillus,Bacillus,Lactobacillus,Exiguobacterium,Brachybacterium,Weissella,Acinetobacter,Enterococcus,Enterobacter,Kocuria,Pediococcus,Aerococcus,Planomicrobium,Desemzia共 18种优势细菌属,Zygosaccharomyces,Wickerhamomyces,Trichosporon,Torulaspora,Thermomyces,Schwanniomyces,Mucor,Monascus,Millerozyma,Microascus,Hyphopichia,Debaryomyces,Clavispora,Candida,Aspergillus共 15种优势真菌属.通过对比制酒晾堂Bacillus,Lactobacilluss,Aspergillus,Wickerhamomyces等 11种优势功能微生物的差异性和变化情况,深入剖析了酱香型白酒第7轮次窖期采用6种方式清理晾堂前后微生物菌群结构及多样性变化.研究建议采用开水浇烫的方式清理晾堂,同时均匀撒60 kg左右大曲粉或10%vol、180 kg左右尾酒于晾堂表面,以增加功能微生物含量.另外,制酒晾堂清理后不宜立即开展生产,适当的时间差有利于功能微生物富集,为新一轮酱香型白酒生产提供适宜的酿造环境.
Research on the Cleaning Methods of the Seventh-Round Airing Court for Jiang Flavour Chinese Spirits
In order to ensure the production of Jiang flavour Chinese spirits,this study systematically investigates the diversity and difference of microbial communities in the aired area and Daqu before the Xiasha round of 2022.The cleaning effectiveness was analyzed from the perspective of microorganisms,focusing on the four experimental processes-pouring boiling water,warming up with scalding hot sorghum,pouring the tail of Baijiu,and splashing Daqu-on the aired area which is used in the traditional process of producing Jiang flavour Chinese spirits.The results showed that a total of 20 bacterial phylum,336 bacterial genera,8 fungal phylum and 138 fungal genera are detected in the aired area and Daqu in the seventh round of 2022.Make it clear 18 genera of dominant bacteria,including Staphylococcus,Oceanobacillus,Ralstonia,Nesterenkonia,Virgibacillus,Bacillus,Lactobacillus,Exiguobacterium,Brachybacterium,Weissella,Acinetobacter,Enterococcus,Enterobacter,Kocuria,Pediococcus,Aerococcus,Planomicrobium,Desemzia.Make it clear 15 genera of dominant fungi,including Zygosaccharomyces,Wickerhamomyces,Trichosporon,Torulaspora,Thermomyces,Schwanniomyces,Mucor,Monascus,Millerozyma,Microascus,Hyphopichia,Debaryomyces,Clavispora,Candida,Aspergillus.By contrast the differences and changes of 11 dominant functional microorganisms in the aired area including Bacillus,Lactobacilluss,Aspergillus,Wickerhamomyces etc.,the changes of microflora structure and diversity were analyzed in depth with 6 ways to clean the aired area of the Jiang flavour Chinese spirits the seventh round.It is recommended to use boiling water to clean the aired area,and evenly sprinkle about 60 kg Daqu powder or 10%vol,180 kg tail wine on the surface of the aired area,which is conducive to increasing the content of functional microorganisms.In addition,the production should not be carried out immediately after the cleaning of the aired area,and the appropriate time difference is conducive to the enrichment of functional microorganisms,which provides a suitable brewing environment for the production of a new round of Jiang flavour Chinese spirits.

Jiang flavour Chinese spiritshigh-throughput sequencingcleaning of the aired areamicrobial diversity

朱治宇、李小波、程艳波、崔同弼、任佳林、黄永涛、沈开田、张启恒

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贵州茅台酒股份有限公司,贵州仁怀 564500

贵州省酿酒工程技术研究中心,贵州仁怀 564500

酱香型白酒 高通量测序 制酒晾堂清理 微生物多样性

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(11)