摘要
以西洋参为原料,多糖得率为主要指标,可溶性固形物为辅助指标,利用纤维素酶和木瓜蛋白酶协同作用优化西洋参酶解条件,进行复合酶比例、复合酶添加量、酶解时间、酶解温度、酶解pH值的单因素实验.在单因素实验的基础上,选择对结果影响较大的酶解时间、酶解温度、酶解pH值作为响应面的自变量进行响应面优化实验确定最佳条件.实验得到的最佳工艺条件为复合酶比例(纤维素酶∶木瓜蛋白酶)1∶3、复合酶添加量1.5％、酶解时间1h、酶解温度50℃、酶解pH6.5,此条件下西洋参汁多糖得率8.30％,可溶性固形物得率41.23％.此方法提取西洋参汁简单温和、效率高,可为西洋参资源开发提供参考.
Abstract
Using American ginseng as the raw material,polysaccharide yield as the main indicator,and soluble solids as the auxiliary indicator,the enzymatic hydrolysis conditions of American ginseng were optimized by the synergistic effect of cellulase and papain.Single factor experiments were conducted on the proportion of composite enzymes,the amount of composite enzymes added,the enzymatic hydrolysis time,the enzymatic hydrolysis temperature,and the enzymatic pH value.Based on the single factor experiment,the enzymatic hydrolysis time,temperature,and pH that have a significant impact on the results were selected as independent variables for the response surface optimization experiment to determine the optimal conditions.The optimal process conditions obtained from the experiment are:composite enzyme ratio(cellulose∶papain)1∶3,composite enzyme addition amount of 1.5％,enzymatic hydrolysis time of 1 hour,enzymatic hydrolysis temperature of 50 ℃,and enzymatic hydrolysis pH of 6.5.Under these conditions,the yield of polysaccharides from American ginseng juice is 8.30％,and the yield of soluble solids is 41.23％.This method for extracting American ginseng juice is simple,mild,and efficient,which can provide reference for the development of American ginseng resources.
维普
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