Enzymatic Hydrolysis Preparation Process of American Ginseng Juice
Using American ginseng as the raw material,polysaccharide yield as the main indicator,and soluble solids as the auxiliary indicator,the enzymatic hydrolysis conditions of American ginseng were optimized by the synergistic effect of cellulase and papain.Single factor experiments were conducted on the proportion of composite enzymes,the amount of composite enzymes added,the enzymatic hydrolysis time,the enzymatic hydrolysis temperature,and the enzymatic pH value.Based on the single factor experiment,the enzymatic hydrolysis time,temperature,and pH that have a significant impact on the results were selected as independent variables for the response surface optimization experiment to determine the optimal conditions.The optimal process conditions obtained from the experiment are:composite enzyme ratio(cellulose∶papain)1∶3,composite enzyme addition amount of 1.5％,enzymatic hydrolysis time of 1 hour,enzymatic hydrolysis temperature of 50 ℃,and enzymatic hydrolysis pH of 6.5.Under these conditions,the yield of polysaccharides from American ginseng juice is 8.30％,and the yield of soluble solids is 41.23％.This method for extracting American ginseng juice is simple,mild,and efficient,which can provide reference for the development of American ginseng resources.
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