首页|基于气相离子迁移谱和气相色谱质谱联用分析红曲酯化酶对苹果醋风味的影响

基于气相离子迁移谱和气相色谱质谱联用分析红曲酯化酶对苹果醋风味的影响

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在苹果醋发酵后期加入红曲酯化酶粗酶制剂,分别采用气相离子迁移谱(Gas chromatography-ion mobility spectroscopy,GC-IMS)和气相色谱质谱联用仪(Gas chromatography-mass spectrometry,GC-MS)对苹果醋风味进行分析.GC-IMS检测分析发现红曲苹果醋中共有36种香气成分含量明显提升,其中有20种物质被定性,包括11种酯类、2种醛类、3种酮类、2种醇类、1种酸类和1种呋喃类物质,且20种物质大多具有令人愉悦的水果香或草木香.GC-MS共检测出47种挥发性香气成分,其中红曲苹果醋中酯类物质含量占比达到75.51%,而对照苹果醋中酯类物质含量仅占44.13%;红曲苹果醋中醇类物质大幅下降,仅占21.66%,而对照苹果醋中醇类物质占到总挥发性香气成分的52.43%.因此,在苹果醋酿造过程中加入红曲酯化酶能够有效提升苹果醋的风味和品质.该研究可为我国苹果醋产业的良好发展提供一定的理论依据.
Analysis of the Effect of Monascus Esterase on the Flavor of Cider Vinegar Based on Chromatography-Ion Mobility Spectrometry and Gas Chromatography-Mass Spectrometry
In the later stage of cider vinegar fermentation,Monascus esterase crude enzyme was added,and the flavor of cider vinegar was analyzed using gas chromatography-ion mobility spectroscopy(GC-IMS)and gas chromatography-mass spectrometry(GC-MS),respectively.GC-IMS analysis found that there was a significantly increased in the content of 36 aroma components in cider vinegar with Monascus esterase crude enzyme,of which 20 substances were identified,including 11 esters,2 aldehydes,3 ketones,2 alcohols,1 acid,and 1 furan substance.Most of the 20 substances had pleasant fruit or plant aromas.A total of 47 volatile aroma components were detected by GC-MS,among which the ester content in cider vinegar with Monascus esterase crude enzyme reached 75.51%,while the ester content in control cider vinegar only accounted for 44.13%.Moreover,the alcohol content in cider vinegar with Monascus esterase crude enzyme decreased significantly,accounting for only 21.66%,while the alcohol content in control cider vinegar accounted for 52.43%of the total volatile aroma components.Therefore,adding Monascus esterase crude enzyme in the process of cider vinegar fermenting can effectively enhance the content of flavor and quality of cider vinegar.This study provide a certain theoretical basis for the development of China's cider vinegar industry.

cider vinegarMonascusesterasegas chromatography-ion mobility spectroscopygas chromatography-mass spectrometry

马艳蕊、孟园、窦勇博、李学震、贾田怡、张鑫、赵岩、刘光鹏

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中华全国供销合作总社济南果品研究所,山东济南 250200

华中农业大学食品科技学院,湖北武汉 430070

苹果醋 红曲霉 酯化酶 气相离子迁移谱 气相色谱质谱联用仪

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(11)