Analysis of the Effect of Monascus Esterase on the Flavor of Cider Vinegar Based on Chromatography-Ion Mobility Spectrometry and Gas Chromatography-Mass Spectrometry
In the later stage of cider vinegar fermentation,Monascus esterase crude enzyme was added,and the flavor of cider vinegar was analyzed using gas chromatography-ion mobility spectroscopy(GC-IMS)and gas chromatography-mass spectrometry(GC-MS),respectively.GC-IMS analysis found that there was a significantly increased in the content of 36 aroma components in cider vinegar with Monascus esterase crude enzyme,of which 20 substances were identified,including 11 esters,2 aldehydes,3 ketones,2 alcohols,1 acid,and 1 furan substance.Most of the 20 substances had pleasant fruit or plant aromas.A total of 47 volatile aroma components were detected by GC-MS,among which the ester content in cider vinegar with Monascus esterase crude enzyme reached 75.51%,while the ester content in control cider vinegar only accounted for 44.13%.Moreover,the alcohol content in cider vinegar with Monascus esterase crude enzyme decreased significantly,accounting for only 21.66%,while the alcohol content in control cider vinegar accounted for 52.43%of the total volatile aroma components.Therefore,adding Monascus esterase crude enzyme in the process of cider vinegar fermenting can effectively enhance the content of flavor and quality of cider vinegar.This study provide a certain theoretical basis for the development of China's cider vinegar industry.