首页|响应面结合模糊数学感官评价法探究泥鳅血管紧张素转化酶抑制肽脱苦工艺

响应面结合模糊数学感官评价法探究泥鳅血管紧张素转化酶抑制肽脱苦工艺

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苦味是限制泥鳅血管紧张素转化酶(Angiotensin converting enzyme,ACE)抑制肽开发的主要因素.文章旨在探究一种良好的脱苦工艺,为泥鳅ACE抑制肽在食品加工、医疗保健等领域的应用提供参考依据.实验采用β-环状糊精脱除泥鳅ACE抑制肽的苦味,结合响应面设计与模糊数学感官评价法对单因素实验结果进一步优化.通过电子舌技术评价泥鳅ACE抑制肽脱苦前后的综合风味,并使用正交偏最小二乘法判别分析对味觉数据进行分析.结果表明,泥鳅ACE抑制肽最佳的苦味脱除工艺参数如下:β-环状糊精的添加量为3.0%、包埋时间30 min、包埋温度40 ℃.在最佳脱苦工艺下,能有效脱除泥鳅ACE抑制肽的苦味,并使苦味回味接近无味点.
Debitter Process of ACE-Inhibiting Peptide of Loach by Response Surface Combined with Fuzzy Mathematical Sensory Evaluation Method
Bitter taste is the main factor limiting the development of angiotensin converting enzyme(ACE)-inhibiting peptides of loach.The purpose of this study is to explore a good process to remove bitterness,hoping to provide a theoretical basis for the application of ACE-inhibiting peptides in food processing,medical care and other fields.β-cyclodextrin(β-CD)was used to remove the bitter taste of ACE-inhibiting peptide of loach.The response surface design combined with fuzzy mathematical sensory evaluation method was used to further optimize the single-factor experimental results.The comprehensive flavor of ACE-inhibiting peptide of loach before and after debitter was detected by electronic tongue technology.The orthogonal partial least squares discriminant analysis(OPLS-DA)was used to analyze the taste data.The results showed that the optimal process parameters for bitterness removal of loach ACE-inhibiting peptides were:β-CD addition of 3.0%,embedding time of 30 minutes,and embedding temperature of 40 ℃.Under the optimal bitterness removal process,the bitterness of loach ACE-inhibiting peptides was effectively removed,and the aftertaste was close to tasteless.

loach ACE inhibitory peptideβ-cyclodextrinresponse surfacefuzzy mathematical sensory evaluationelectronic tonguebitter taste removal

顾嘉慧、曹园、邵娟娟

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河北农业大学理工学院,河北沧州 061000

泥鳅ACE抑制肽 β-环状糊精 响应面 模糊数学感官评价法 电子舌 苦味脱除

2024

食品科技
北京市粮食科学研究所

食品科技

CSTPCD北大核心
影响因子:0.622
ISSN:1005-9989
年,卷(期):2024.49(11)