Optimization of Polysaccharide Production from Edible and Medicinal Fungi via Solid-State Fermentation of Lentinula edodes Stem Substrate and Its Analysis of Antioxidant Activity
The study investigated the optimal process for producing polysaccharides from edible and medicinal fungi via solid-state fermentation of Lentinula edodes stem substrate and its antioxidant activity.Polysaccharides were prepared by fermentation of Lentinula edodes stem by Morchella,Poria cocos,Stropharia rugosoannulata,Volvariella volvacea,Lentinula edodes,Ganoderma lucidum,Lepista sordida,Schizophyllum commune,Hericium erinaceus,Tremella sanguinea Peng and other fungi.The optimal fungal was selected,and both single-factor and orthogonal experiments were conducted to optimize the preparation process.The antioxidant activity of the polysaccharides obtained before and after fermentation was evaluated by the 1,1-diphenyl-2-picrylhydrazyl(DPPH)radicals scavenging assay.The results revealed that Schizophyllum commune was the optimal fermentation strain.The optimal conditions for solid-state fermentation of Lentinula edodes stem substrate(10 g)to produce polysaccharides were determined to be a fermentation period of 9 days,inoculum volume of 12.5 mL,and amount of water added of 1∶1.5(g/mL).Under these conditions,the polysaccharide yield reached(16.80±0.60)％,with a purity of(75.40±1.81)％.The polysaccharides obtained by fermenting had a stronger scavenging effect on DPPH radical,and its IC50 value was 2.445 mg/mL.Solid-state fermentation of Lentinula edodes stem by Schizophyllum commune improved polysaccharide yield,purity,and antioxidant activity.These findings provide a theoretical basis for the preparation of polysaccharides from Lentinula edodes stem and contribute to the development of its resources.
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