Real-Time Polymerase Chain Reaction Assay for Detection of Cassava Ingredients in Pure Sweet Potato Vermicelli
Objective:In order to detect adulteration in pure sweet potato vermicelli from market,a qualitative detection method was developed in this paper for real-time fluorescence polymerase chain reaction(PCR).Method:The effective amplified concentration of DNA was collected by enrichment,the specificity and adaptability of this method were tested,and the cassava ingredients were detected in 66 pure sweet potato vermicelli samples.Results:The detection result showed that cassava components were detected in 13 batches of 66 actual samples.Conclusion:This method realized the qualitative detection of the cassava ingredients in pure sweet potato vermicelli,providing strong technical support for law enforcement and regulatory authorities.
pure sweet potato vermicellireal-time polymerase chain reactionDNA extractioncassava ingredients
万方数据
维普
