实时荧光聚合酶链式反应法检测纯红薯粉条中的木薯成分

Real-Time Polymerase Chain Reaction Assay for Detection of Cassava Ingredients in Pure Sweet Potato Vermicelli

邓迎春 ¹刘颖 ¹苏利霞 ¹郭旭光 ¹陶健¹

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作者信息

1. 河南省食品和盐业检验技术研究院,国家市场监管重点实验室(食品安全快速检测与智慧监管技术),河南郑州 450003
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摘要

目的:为了对市售纯红薯粉条掺假检测,文章建立了实时荧光聚合酶链式反应法(Polymerase chain reaction,PCR)检测纯红薯粉条中木薯成分.方法:通过富集的方式收集到有效扩增浓度的DNA,测试了该方法的特异性和适应性,并对抽取的66份纯红薯粉条样本进行木薯成分的检测.结果:在66份实际样本中共检测出13批样本存在木薯成分.结论:该方法可实现对纯红薯粉条掺假的定性检测,为执法监管部门提供有力的技术保障.

Abstract

Objective:In order to detect adulteration in pure sweet potato vermicelli from market,a qualitative detection method was developed in this paper for real-time fluorescence polymerase chain reaction(PCR).Method:The effective amplified concentration of DNA was collected by enrichment,the specificity and adaptability of this method were tested,and the cassava ingredients were detected in 66 pure sweet potato vermicelli samples.Results:The detection result showed that cassava components were detected in 13 batches of 66 actual samples.Conclusion:This method realized the qualitative detection of the cassava ingredients in pure sweet potato vermicelli,providing strong technical support for law enforcement and regulatory authorities.

关键词

纯红薯粉条/实时荧光聚合酶链式反应法/DNA提取/木薯成分

Key words

pure sweet potato vermicelli/real-time polymerase chain reaction/DNA extraction/cassava ingredients

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出版年

2024

食品科技

北京市粮食科学研究所

食品科技

CSTPCD北大核心

影响因子：0.622

ISSN：1005-9989

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