Determination of Oxyphenisatine,Phenolpthalein and Their Derivatives in Food by UPLC-DAD-Q-TOF-MS/MS
Objective:To establish a method by ultra-high performance liquid chromatography/quadrupole time-of-flight-mass spectrometry(UPLC-DAD-Q-TOF-MS/MS)for the detection of 24 kinds of oxyphenisatine,phenolpthalein and their derivatives in food.Methods:The analysis was performed using an UPLC-DAD-Q-TOF-MS/MS system with a Waters HSS T3 1.8 μm C18 column.The mobile phase consisted of 0.1％formic acid and a mixture of methanol and acetonitrile(1∶1)for gradient elution(0 min,20％A;6 min,95％A;10 min,95％A;10.5 min,20％A;12 min,20％A).The flow rate was set to 0.3 mL/min.Target MS/MS mode was used with electron spray ionization(ESI)positive ionization,and the scanning range was 50-1000.Results:The method showed good linearity in the concentration range of 0-50μg/mL,with relative standard deviations(RSD％)less than 3％.The recovery rates of the components ranged from 84.2％to 97.7％,and the method demonstrated good stability.One batch of samples detected oxyphenisatin diacetate impurity 2 with a content of 2573 mg/kg,and two batches detected phenolphthalein,with contents of 1538 mg/kg and 2054 mg/kg,respectively.Conclusion:This method is fast,accurate,and highly sensitive.It further enhances the detection of illegal chemical additives in weight loss and intestinal laxative foods and provides technical support for ensuring food safety.
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维普
