Determination of Tricaine Methanesulfonate and Eugenol in Freshwater Fish by Ultra-High Performance Liquid Chromatography Tandem Mass Spectrometry
Objective:To establish an analytical method for the determination of tricaine methanesulfonate and eugenol in freshwater fish using ultra-high performance liquid chromatography tandem-mass spectrometry.Methods:The sample was extracted from the centrifuge and purified by HRM solid-phase extraction column,then passed through a polytetrafluoroethylene membrane,and determined by ultra-high performance liquid chromatography tandem mass spectrometry.The chromatographic column was an ACQUITY UPLC BEH C8 column(2.1 mm×100 mm,1.7 μm),using water-methanol as the mobile phase and adopting the isocratic elution mode,the electrospray ion source(positive and negative ion scanning)mode and multiple reaction monitoring(MRM)mode were selected for mass spectrometry analysis.Results:The linear range of tricaine was 0.1-20 ng/mL,and that of eugenol was 1.0-200 ng/mL,with correlation coefficients(r)greater than 0.999.The limit of detection(LOD)for tricaine was 0.1 μg/kg and the limit of quantification(LOQ)was 0.3 μg/kg,the LOD for eugenol was 1.0 μg/kg and the LOQ was 3.0 μg/kg.The recovery rates at low,medium,and high spiked levels ranged from 87.9％to 114％,with relative standard deviations(RSD)between 1.47％and 8.46％.Conclusion:The isotope-labeled internal standard method using liquid chromatography-tandem mass spectrometry was successfully applied to simultaneously determine tricaine and eugenol in freshwater fish.This method is highly sensitive,accurate,exhibits good linearity and separation,and is suitable for the simultaneous detection of tricaine and eugenol in freshwater fish.
ultra-high performance liquid chromatography tandem mass spectrometrytricaine methanesulfonateeugenolfreshwater fish
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维普
