Research on Pre-Treatment for Detection of Poppy Shell Components in Soup
Objective:To compare the differences in the use of different purification filter materials for surface enhanced Raman spectroscopy(SERS)and ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS-MS)determination of poppy shell components in food soup.Methods:UPLC-MS-MS adopted ACQUITY UPLC®BEH HILIC C18 chromatography column,gradient elution of 10 mmol/L ammonium acetate solution with 0.1％formic acid and 0.1％formic acid acetonitrile solution at a flow rate of 0.3 mL/min.A laser wavelength of 785 nm and a laser power of 250 mW were used in SERS method.Result:In the UPLC-MS/MS method,the average recovery rates for 5 components in poppy shell were as follows:65.4％to 97.1％for self-mixed filter media,69.3％to 87.9％for CQ11-3 filter media,43.4％to 109.8％for 6550HP filter media,72.7％to 103.8％for 3150-VD filter media,85.7％to 102.6％for 3150-VDS filter media,12.4％to 104.3％for QVet-NM+filter media,23.2％to 102.1％for QVet-HP+filter media,69.6％to 86.5％for AOAC filter media,74.0％to 92.5％for Captiva EMR-Lipid filter media,64.2％to 80.8％for EMR-GPD with Carbon S filter media,and 73.6％to 98.2％for Styra MCX filter media.In the SERS method,the detection limits for QuEChERS filter materials CQ11-3 and AOAC,push-type QuEChERS filter materials 3150-VDS and 3150-VD,and solid phase extraction(SPE)column filter materials Styra MCX and Captiva EMR-Lipid were 40-80 μg/kg,while the detection limits for other filter materials were 160 μg/kg.The average test time for self-mixed and QuEChERS filter materials was approximately 9.75 minutes,the average test time for push-type QuEChERS filter materials was approximately 6-7 minutes,and the average test time for SPE column filter materials was approximately 18 minutes.Conclusion:The push-type QuEChERS combined with the SERS method enables the rapid detection of five alkaloid compounds in poppy shells in soup stock.The detection limits and accuracy meet the required detection standards.If the result is positive,the UPLC-MS-MS method can be used for confirmation.
万方数据
维普
