Progress of Isothermal Amplification Technology for Dairy Safety Detection
Milk and dairy products come in a wide variety and are highly favored by consumers.However,the booming development of the dairy industry has brought about safety issues.Contamination of dairy products by pathogenic microorganisms is one of the primary causes of these safety concerns,necessitating effective detection methods for their identification and control.Currently,commonly used detection methods include culturing techniques and polymerase chain reaction(PCR).However,culturing methods are time-consuming and labor-intensive,while traditional PCR techniques require relatively sophisticated equipment.Isothermal amplification techniques,with their lower equipment requirements,shorter detection time,simplicity of operation,and cost-effectiveness,hold great promise as an important method for detecting foodborne pathogens.This article introduces the types of pathogens affecting dairy safety,elaborates on the classification of isothermal amplification techniques and their application progress in dairy products,compares major isothermal amplification methods,and discusses future research directions,aiming to provide a theoretical basis for the detection of harmful microorganisms in dairy products.
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维普
