分别采用邻苯三酚-鲁米诺化学发光体系、硫酸亚铁-鲁米诺-过氧化氧化学发光体系和过氧化氧-鲁米诺化学发光体系测定白腊、妃子笑、糯米糍和怀枝4个品种的荔枝皮原花青素(procyanidins of litchi pericarp,LPPC)体外清除超氧阴离子自由基、羟自由基和过氧化氧的能力.同时运用硫酸铜-邻非哕啉-抗坏血酸-过氧化氧-DNA体系测定不同LPPC对诱导体系DNA损伤的保护作用.实验结果表明:白腊LPPC具有较好的体外清除超氧阴离子自由基和过氧化氢的能力;妃子笑呈现出较显著的羟自由基清除能力和保护DNA损伤的效果.不同品种的LPPC在不同化学发光体系中的抗氧化活性呈现较大差异.
Antioxidant Activity and DNA Damage Preventing Effect of Procyanidins from Litchi chinensis Pericarp
In order to evaluate antioxidant activity in vitro of procyanidins from pericarp of four cultivars (Baila, Feizixiao, Nuomizi, Huaizhi) of litchi (LPPC), superoxide anion radical, hydroxyl radical and hydrogen peroxide scavenging assays were carried out using pyrogallo-luminol, ferrous sulfate-Luminol-hydrogen peroxide and Luminol-hydrogen peroxide systems, respectively. In addition, copper sulfate-phen-VC-hydrogen peroxide-DNA system was used to determine the preventing effect of LPPC against DNA damage. Results showed that among the litchi cultivars, Baila LPPC possessed the best scavenging potency against superoxide anion and hydrogen peroxide and Feizixiao LPPC indicated the most efficient hydroxide radical scavenging ability and DNA damage preventing effect. In conclusion, procyanidins from pericarp of different litchi cultivars acted different antioxidant effects in different chemiluminescence systems.
LPPCchemiluminescence methodantioxidant activity in vitroDNA damage prevention
国家科技期刊平台
NSTL
万方数据
维普
