Optimization of SF-CO_2 Extraction of Essential Oil from Seeds of Evening Primrose(Oenothera biennis)Grown in West Hunan Province by Response Surface Methodology
Seeds of evening primrose variety Laer Mountain No.1 grown in west Hunan province were used as the raw material to extract essential oil in a supereritical fluid supercritical CO_2 extraction apparatus.A series of central composite design experiments was conducted for developing a mathematical regression model for oil yield as a response of four variables(namely extractionpressure,extraction temperature,separation temperature,and extraction time).Besides,based on this,response surface and contour plots were drawn for analyzing the interactive effects of the above variables on oil yield.Results indicated that the optimal extraction process was obtained as follows:39 MPa of extraction pressure,extraction temperature of 43 ℃,separation temperature of 40℃,70 min of extraction time,which resulted in a maximum oil yield of 20.07%.