Optimization of Extraction Technology of Polysaccharides from Petasites tricholobus Roots by Response Surface Analysis
Crude polysaccharides were prepared from the residue of Petasites tricholobus roots after flavonoids extraction.Accordmg to single-factor experiments,three levels of the factors including extraction temperature,extraction time and liquid/solid ratio were selected for the central composite design to establish a quadric regression equation for predicting the yield of polysaccharides.By response surface analysis,the optimum extraction conditions were obtained as follows:extraction temperature 70℃,extraction time 2.3 h and liquid/solid ratio 45:1,which led to estimated and observed values of maximal yield of polysaccharides of 35.04% and 34.74%,respectively.
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