首页|海带岩藻多糖的水提制备及其抗氧化活性研究

海带岩藻多糖的水提制备及其抗氧化活性研究

扫码查看
以热水浸提后乙醇沉淀的方法从海带中提取制备岩藻多糖.通过单冈素试验和正交优化试验确定最佳提取工艺,可获得岩藻多糖最大提取率为4.99%,硫酸-苯酚法测定多糖的含量为96.9%.多糖提取物对羟自由基、超氧阴离子自由基的清除作用,还原力测定以及在模拟胃液条件下对NO_-~2的清除实验结果表明,海带中岩藻多糖提取物具有较强的体外抗氧化性能,且随着多糖质量浓度的增大,其抗氧化活性整体呈逐渐增强趋势.
Water Extraction and Antioxidant Activity of Fucoidan from Laminaria japonica
Hot water extraction followed by ethanol precipitation was used for production of fucoidan from Laminaria japonica in this study.To maximize fucoidan yield,the optimal values of crucial parameters of hot water extraction,including extraction temperature and time and material/liquid ratio were determined by single factor and orthogonal array methods to be 70℃,6 h and 1:40,respectively.Under the above conditions,the maximum yield of fucoidan with 96.9% purity was up to 4.99%.Laminaria japonica fucoidan had strong antioxidant performance in vitro,which was reflected by hydroxyl and superoxide anion free radical scavenging activities,reducing power and nitrite scavenging activity under simulated gastric environment,and its antioxidant performance in vitro exhibited a gradual increase with its increasing concentration.

fucoidanLaminaria japonicaextractionantioxidant

程仕伟、陈超男、冯志彬、张玉香

展开 >

应用生物技术山东省重点实验室,鲁东大学生命科学学院,山东,烟台,264025

岩藻多糖 海带 提取 抗氧化

鲁东大学科研基金

LY20083305

2010

食品科学
北京食品科学研究院

食品科学

CSTPCDCSCD北大核心
影响因子:1.327
ISSN:1002-6630
年,卷(期):2010.31(6)
  • 28
  • 7