Microbial Diversity of Tianyuan Jiangyuan Soybean Paste during Fermentation as Analyzed by PCR-DGGE
The microbial diversity of Tianyuan Jiangyuan soybean paste was analyzed by PCR-DGGE (polymerase chain reaction- denaturing gradient gel electrophoresis) technique.The results showed that Bacillus licheniformis (98%) and the closely related species of uncultured Leuconostoc sp.clone (100%) were the dominant bacteria during soybean paste fermentation.Bacillus assisted with fungi in decomposing proteins and starches in the raw materials.Leuconostoc sp.contributed to the formation of flavor compounds in soybean paste.The closely related species ofAspergillus oryzae (99%) were the dominant fungi during soybean paste fermentation.It mostly decomposed proteins and starches in the raw materials.