建立电导抑制离子色谱法测定八角中莽草酸含量的方法.用Metrohm A supp 4(4mm × 250mm)型阴离子分析柱,以1.8mmol/L Na2CO3+1.7mmol/L NaHCO3作淋洗液,淋洗液流速为0.8mL/min,分析时间15min.在优化色谱条件下,莽草酸在1～100mg/L范围内,质量浓度与峰面积有良好线性关系(r=0.9993),检出限为0.02～0.8mg/L.连续6次进样,峰面积的相对标准偏差为1.04%,保留时间的相对标准偏差为0.47%,样品加标回收率为97.3%～98.9%.该方法快速,样品前处理简单,结果满意.
Determination of Shikimic Acid in Chinese Star Anise Fruit by Ion Chromatography with Suppressed Conductivity Detection
A method for the determination of shikimic acid in Chinese star anise (Illicium verum Hook. f.) fruit by ion chromatography with suppressed conductivity detection was developed. The chromatographic separation was conducted on Metrohm A supp 4 (4 mm × 250 mm) anion exchange column, using 1.8 mmol/L Na2CO3 + 1.7 mmol/L NaHCO3 as a mobile phase at a flow rate of 0.8 mL/min. The total analysis time was 15 min. The linear range was between 1 mg/L and 100 mg/L and the correlation coefficient was 0.9993. A detection limit of 0.02～0.8 mg/L was achieved under optimized separation conditions.The RSD of peak area was 1.04％ (n = 6), and the RSD of retention time was 0.47％. The average spike recoveries were between 97.3％ and 98.9％. This method is fast, simple and reliable.
国家科技期刊平台
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万方数据
维普
