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杨梅叶中总黄酮提取工艺及其抗氧化活性研究

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采用用正交试验优化杨梅叶中总黄酮的最佳提取工艺条件。以杨梅叶为原料,在乙醇体积分数65%、料液比1:30(g/mL)、提取时间1.5h、提取次数3次条件下,总黄酮提取率为3.34%。测定杨梅叶中总黄酮的体外抗氧化活性活性,结果表明:杨梅叶总黄酮对羟自由基、超氧阴离子自由基均有较强的清除作用,是一种天然有效的抗氧化剂。
Extraction and Antioxidant Activity Evaluation of Total Flavonoids from Bayberry(Myrica rubra Sieb.et Zucc.) Leaves
An L9(34) orthogonal array design was employed to optimize the extraction of total flavonoids from bayberry(Myrica rubra Sieb.et Zucc.) leaves.The in vitro antioxidant activity of total flavonoids extracted was evaluated by determining their scavenging capacity against hydroxyl radicals and superoxide anions.The optimum extraction conditions were determined as follows: 65% aqueous ethanol solution as extraction solvent at a material/liquid ratio of 1:30(g/mL) for triple repeated extractions for 1.5 h each time and the extraction rate is 3.34%.Total flavonoids from bayberry leaves could strongly scavenge hydroxyl and superoxide anion free radicals,thus being a powerful natural antioxidant.

bayberry leavestotal flavonoidsextraction conditionsantioxidant activity

林建原、陶志华、朱钐汕

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浙江万里学院生物与环境学院,浙江宁波315100

杨梅叶 总黄酮 提取工艺 抗氧化活性

浙江省自然科学基金

Y2110865

2011

食品科学
北京食品科学研究院

食品科学

CSTPCDCSCD北大核心
影响因子:1.327
ISSN:1002-6630
年,卷(期):2011.32(20)
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