Comparison of Three Chromatographic Columns in Separation and Analysis of 37 Fatty Acids in Fish Oil
The separation performance of columns with different polarities,including HP-5,DB-Wax and SPB-50 column,for 37 fatty acid methy esters（FAMEs） was compared by gas chromatography-mass spectrometry（GC-MS）.The results showed that these columns allowed FAMEs separation.The peaks of FAMEs with less carbon atoms were separated earlier.FAMEs with the same number of carbon atoms and different degree of saturation were separated in different orders on the stationary phases.SPB-50 column could separate cis/trans,ω-3 and ω-6 fatty acids more sufficiently and may be a more suitable column for complicated FAME sample analysis,especially for functional fatty acids.
国家科技期刊平台
NSTL
万方数据
维普
