Optimization of Extraction Process for Proanthocyanidins from Fruits of Caryota mitis Lour
The extraction of proanthocyanidins from Caryota mitis fruits was optimized by orthogonal array design. The raw material was freeze-dried, comminuted, sieved through an 80-mesh sieve and extracted by solvent extraction technique. The resultant extract was determined by butanol-HCl method for absorbance at 546 nm. The optimum conditions for the extraction of proanthocyanidins were determined as two extraction cycles with 60% ethanol aqueous solution alkalified to pH 9 at a solid/solvent ratio of 1:15 (g/mL) for 2.5 h at 50 ℃. Under these conditions, the extraction rate of proanthocyanidins was 95.18%. In the fifth repeated extraction, the extraction rate of proanthocyanidins was 6.59%. This study demonstrates that Caryota mitis fruits are rich in proanthocyanidins and therefore has great development potential.
Caryota mitis Lour. fruitproanthocyanidinsextraction process
国家科技期刊平台
NSTL
万方数据
维普
