Detection for Pig-Derived Components in Foods by Real-Time Polymerase Chain Reaction
Species-specific real-time PCR (TaqMan) assay was developed for the detection of pig-derived components in foods.Primers and Taqman probe of this assay were designed with pig conservative regions of the mitochondrial cytochrome b (Cytb) gene (amplicon =98 base pairs).The specificity was evaluated and achieved without amplification on DNA from other meats.Results showed that this method revealed a high sensitivity and could detect 1 pg of pork template DNA, while Ct (cycle threshold) values of negative samples were limited to 35 cycles.Applying this assay method, the recovery rate of DNA extracted from meat mixtures was up to 1％ pork spiked in other species.Therefore, it is a potentially reliable and suitable technique in routine food analysis for the detection of pork in foods.
real-time polymerase chain reactionpigmitochondrial cytochrome b genefood adulteration
国家科技期刊平台
NSTL
万方数据
维普
