Real-Time PCR Assay for Detection of Banana Ingredients in Foods
Specific primers and probe were designed according to the highly conserved sequence of ribulose-1,5-bisphosphate carboxylase (rbcL) gene in banana.A real-time fluorescence polymerase chain reaction (real-time PCR) assay for banana ingredients in foods was developed.The probe and primers were highly specific to banana,and resulted in no positive reaction signal when applied to 55 other kinds of food materials.The detection limit of the method was 0.0001 ng/μL for banana DNA and 0.001％ (m/M) for banana powder.All these results confirm that the real-time PCR assay is a rapid,sensitive and specific method for detecting banana ingredients in foods.
国家科技期刊平台
NSTL
万方数据
维普
