Bioactive Component Content and Antioxidant Activity of Chrysanthemum Flower (Chrysanthemum morifolium Ramat) Extract
Objective:To establish a method for determining the contents of major bioactive components in chrysanthemum flower and evaluate the antioxidant activity of chrysanthemum flower extract.Methods:HPLC was used to determine the contents of chlorogenic acid,luteoloside,quercetin,luteolin and apigenin in chrysanthemum flower extract.Total flavonoids and antioxidant activity of chrysanthemum flower extract were determined to explore the correlation between both indexes.Results:Bioactive components in different chrysanthemum cultivars were different.The highest level of chlorogenic acid was observed in Gong Ju,which was 9.8 mg/g.The highest levels of luteoloside and quercetin were detected in Hangbai Ju,which were 0.94 mg/g and 2.76 mg/g,respectively.The highest level of luteolin was determined in Bo Ju,which was 2.48 mg/g.The highest level of apigenin was observed in Chu Ju,which was 0.20 mg/g.The total flavonoids in Gongju and Hangbai Ju were 5.07％ and 2.65％,respectively.Compared with Hangbai Ju flower extract and commercial chrysanthemum flower extract,Gongju flower extract had higher T-AOC and scavenging activity against hydroxyl and DPPH free radicals,which were (51.22 ± 1.66) U/mg,(57.38 ± 1.39) U/mg and (89.68 ± 0.23)％,respectively An excellent correlation between antioxidant properties and total flavonoids content was observed (R2 =0.993,P ＜ 0.05 for T-AOC; R2 =0.968,P ＜ 0.05for hydroxyl free radical scavenging activity; R2 =0.977,P ＜ 0.05 for DPPH free radical scavenging activity).Conclusion:Chrysanthemum morifolium Ramat flowers from different cultivars have different bioactive components.Gongju has the strongest antioxidant activity,which is related to total flavonoids.
国家科技期刊平台
NSTL
万方数据
维普
