Isolation, Identification and Preliminary Screening of Acid-Tolerant Lactic Acid Bacteria from Naturally Fermented Pickle Juices from Daqing
Fourteen strains of suspected lactic acid bacteria were isolated from 7 naturally fermented pickle juices gathered from Daqing,Heilongjiang Province.16S rDNA was extracted from each of these bacterial strains using a bacterial genomic DNA extraction kit.The species were identified by DNA sequencing,homology analysis and phylogenetic tree.The results indicated that the all 14 strains belonged to lactic acid bacteria,including 4 strains of Lactobacillus curvatus (HD12-1,HD13-5,HD14-1 and HD15-1),1 strain of Lactobacillus brevis (HD18-2),3 strains of Lactobacillus sakei (HD12-2,HD13-1 and HD16-5),1 strain of Leuconostoc mesenteroides (HD18-3),and 5 strains of Lactobacillus plantarum (HD14-3,HD15-2,HD16-2,HD17-3 and HD17-4).Among these strains,6 strains,including HD12-1,HD13-1,HD14-1,HD15-1,HD16-2 and HD16-5,were able to grow at pH 2.5,3.0 or 3.5,with a survival rate above 2％ at pH 3.0.
国家科技期刊平台
NSTL
万方数据
维普
