Optimization of Enzymatic Pretreatment Process for Pressing of Evening Primrose Oil
In this work,evening primrose oil was pressed from enzymatically pretreated samples of evening primrose seeds.The enzymatic pretreatment was carried out using a mixture composed of a commercial complex enzyme preparation hydrolyzing carbohydrates,viscozyme and cellulase (all from Novozymes,4∶1∶1).By using one-factor-at-a-time and orthogonal array design methods,the optimal enzymatic pretreatment conditions were determined to be hydrolysis for 5.5 h at 50 ℃,initial pH 5.0 with a total enzyme dosage of 2％.Under these conditions,the yield of free oil was 24％.The pretreated Material was dried until the moisture content reached 9％,and then pressed initially at 60 ℃,finally at 90 ℃ and 3.5 MPa,resulting in an oil yield of 69％.γ-Linolenic,linoleic and some other unsaturated fatty acids were retained at significant levels in the evening primrose oil,and the oil quality was good.
evening primrose oilenzymatic methodpressingoil yielddetermination of quality parameters
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