Effect of Isoquercetin from Fagopyrum tataricum on the Proliferation and Apoptosis of Human Gastric Carcinoma Cell Line SGC-7901
Isoquercetin was extracted from Fagopyrum tataricum and investigated for its effect on cell proliferation,apoptosis and migration of human gastric carcinoma cell line SGC-7901.Cell proliferation was examined by using MTT assay.The morphological changes of SGC-7901 were observed by DAPI nuclear staining.The effect of Fagopyrum tataricum isoquercetin on cell migration was investigated by scratch-recovering tests in cell culture plates,as well as its effect on cell apoptosis by flow cytometry.As a result,MTT assay showed that the isoquercetin could inhibit the viability of SGC-7901 cells in time-and dose-dependent manners.SGC-7901 cell growth was inhibited by 35.92％ or more at isoquercetin concentration of 100 μmol/L for 48 h,compared to only 3.15％ for 293T cells under the same conditions of concentration and culture duration.DAPI nuclear staining showed chromosomal condensation and the production of apoptotic bodies.Scratch-recovering tests indicated that the isoquercetin could suppress cell migration.Flow cytometry showed that the number of cells at the G1 phase and S phase decreased and increased at the G2/M phase and the apoptotic cell population exhibited a significant increase.Taken together,isoquercetin from F.tataricum can induce apoptosis,arrest cell cycle and inhibit cell proliferation and migration of SGC-7901 cells.
isoquercetinhuman gastric carcinoma cell line SGC-7901cell cycleapoptosiscell migration
国家科技期刊平台
NSTL
万方数据
维普
