首页|酶解法提取罗非鱼血液中血红素的工艺条件优化

酶解法提取罗非鱼血液中血红素的工艺条件优化

扫码查看
原文链接
  • 国家科技期刊平台
  • NETL
  • NSTL
  • 万方数据
  • 维普
为更加科学有效地利用丰富的罗非鱼血液资源,研究酶解法提取罗非鱼血液中血红素的工艺条件,通过单因素试验和正交试验确定了复合酶提取血红素的最佳酶解条件为:温度40℃、pH 8.0、底物质量浓度6 g/100 mL、酶添加量8 000 U/g、酶解时间2h.再经过热处理、酸沉淀和真空冷冻干燥等纯化工艺后得血红素终产品.在此条件下,血红素提取率达到80.9％,产品纯度为28.2％.该工艺简单可行,生产成本低,适合工业化生产,不仅能有效地减少环境污染,还能显著地增加企业产品的附加值.
Optimization of Extraction Conditions of Heme from Tilapias Blood by Enzymatic Hydrolysis
To achieve scientific and effective utilization of tilapias blood as an abundant resource in China,the extraction conditions of heme from tilapias blood by enzymatic hydrolysis were studied in this paper.Protamex was found as the best enzyme catalyst for the extraction of heme from tilapias blood.By using single-factor and orthogonal array designs,the optimum enzymatic hydrolysis conditions were determined as follows:temperature,40 ℃; pH,8.0; substrate concentration,6 g/100 mL; enzyme/substrate,8 000 U/g; and hydrolysis time,2 h.Under these conditions,followed by heat treatment,acid precipitation and vacuum freeze drying,heme was obtained with a yield of 80.9％ and a purity of 28.2％.This extraction procedure is simple,practical,cost saving and suitable for industrial production of value-added products with reduced environmental pollution.

tilapias bloodhemeenzymatic hydrolysisextraction

岑剑伟、李来好、杨贤庆、刘在军、郝淑贤、周婉君、魏涯

展开 >

中国水产科学研究院南海水产研究所,农业部水产品加工重点实验室,国家水产品加工技术研发中心,广东广州 510300

上海海洋大学食品学院,上海 201306

罗非鱼血 血红素 酶解 提取

国家现代农业产业技术体系建设专项“十二五”国家科技支撑计划项目国家农业科技成果转化资金项目国家农业科技成果转化资金项目广东省科技计划项目广西科学研究与技术开发计划项目

CARS-492012BAD28B062010GB23260577 2010GB2E0003352009A02070000411107005-2

2014

10.7506/spkx1002-6630-201416006

食品科学
北京食品科学研究院

食品科学

CSTPCDCSCD北大核心
影响因子：1.327
ISSN：1002-6630
年,卷(期)：2014.35(16)
  • 6
  • 12