Correlation between Total Phenolic Content and Antioxidant Activity in Apple Seeds from Seven Cultivars
This study aimed to investigate the correlation between the total phenolic content and antioxidant activity in apple seeds from seven cultivars.The total phenolic content was determined by Folin-Ciocalteu method,the antioxidant activity was evaluated by total reducing capacity,3-ehtylbenzothiazolin-6-sulfnic acid diammonium salt (ABTS) scavenging,superoxide anion scavenging,hydroxyl free radical scavenging and 1,l-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging assays,and the polyphenol composition of apple seeds was analyzed by high performance liquid chromatography (HPLC).It was shown that Honeycrisp exhibited the highest total phenolic content and the best antioxidant properties among seven apple cultivars.Antioxidant activity of polyphenols in apple seeds was significantly positively correlated with total phenolic content (r =0.771-0.984),with the exception of DPPH scavenging capacity.Compared to tea polyphenols,vitamin C (VC) and 2,6-di-tert-butyl-4-methylphenol (BHT),apple seed polyphenols exhibited better antioxidant activity.Phloridzin,which accounted for 79.76％-86.67％ of the total phenolic content as determined by HPLC,was the dominant antioxidant component in apple seeds.Polyphenols extracted from apple seeds could have a wide application prospect because they act as the safe,non-toxic natural antioxidants.
国家科技期刊平台
NSTL
万方数据
维普
