摘要
本实验以白鲢鱼皮为原料提取鱼皮明胶,考察不同提取温度(30、50、70、90、100℃)对鱼皮明胶得率和理化性质的影响.结果表明:不同温度条件下提取的鱼皮明胶的紫外吸收峰均在波长218 nm左右;明胶提取率在提取温度为90℃时最大,为(86.91±0.98)％;50℃条件下提取的鱼皮明胶的凝胶强度最大,为(896.75±117.03)g;聚丙烯酰胺凝胶电泳图谱显示,30、50℃条件下提取的鱼皮明胶由α1、α2、β3条肽链组成,70、90、100℃条件下提取的鱼皮明胶由于明胶分子的热降解,电泳条带不明显;30、50、70、90、100℃条件下提取的鱼皮明胶的热变性温度分别为(97.88±2.65)、(108.66±0.43)、(106.48±3.33)、(100.27±2.37)、(99.56±0.37)℃;提取温度越高,明胶的G"和G'值越小、流变性能越差.
Abstract
Gelatin was extracted from silver carp (Hypophthalmichthys molitrix) skin,and the physical and chemical characteristics of gelatin extracted at different temperatures (30,50,70,90 and 100 ℃) were compared.The maximum ultraviolet absorption peaks of all gelatin appeared near 218 nm.The maximum gelatin recovery ((86.91 ± 0.98) ％) was obtained when extraction was performed at 90 ℃,while the maximum gel strength ((896.75 ± 117.03) g) was obtained when the extraction temperature was 50 ℃.The SDS-PAGE pattern indicated that gelatin extracted at 30 and 50 ℃ contained a1,a2 and β chains,while that extracted at 70,90 and 100 ℃ did not have obvious bands,which was presumably ascribed to heat degradation of gelatin.The thermal denaturation temperatures of the gelatin extracted at 30,50,70,90 and 100 ℃ were (97.88 ± 2.65),(108.66 ± 0.43),(106.48 ± 3.33),(100.27 ± 2.37) and (99.56 ± 0.37) ℃,respectively.The higher the extraction temperature was,the smaller the values of G' and G" were,and the worse rheological properties of gelatin were.
基金项目
安徽省“115”产业创新团队项目(2012d5t146)
国家科技期刊平台
NSTL
维普
万方数据