Optimization of Pectinase-Assisted Extraction of Proanthocyanidin from Cynomorium songaricum Rupr. Using Response Surface Methodology
Purpose: To optimize the pectinase-assisted extraction of proanthocyanidins (PC) from the stems of Cynomorium songaricum Rupr.. Methods:Pectinase was introduced into the process for improving the extraction efficiency. The extraction conditions were optimized by using response surface methodology (RSM). Five key factors including enzyme dosage, hydrolysis duration, pH value, temperature and ethanol concentration were selected by one-factor-at-a-time experiments. The extraction yield of PC was used as the response. Results:A quadric regression equation for predicting the extraction yield of PC was established using Box-Behnken design and the extraction conditions were optimized as follows:hydrolysis for 34 min with 1%pectinase at 52℃and initial pH 4.8 followed by extraction for 1.5 h with 70%ethanol as the extraction solvent. Under these optimized conditions, the extraction yield of PC reached 14.30%. Conclusion:The optimized pectinase-assisted extraction method can enhance the extraction efficiency of PC from Cynomorium songaricum Rupr..
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