Optimization of Extraction and Structural Analysis of Polysaccharide from Paecilomyces hepiali Chen& Dai
Response surface methodology was applied to optimize polysaccharide extraction from Paecilomyces hepiali Chen & Dai.The extracted polysaccharide was structurally elucidated.The optimal extraction conditions were found to be 4.5 h extraction with hot water at 98.0 ℃ at water/material ratio of 25∶1 (mL/g).Under these conditions,the maximum polysaccharide yield of 9.69％ was obtained and the polysaccharide mainly composed of neutral polysaccharide (84.7％) and uronic acid (9.5％).High performance size exclusion chromatography (HPESC),high performance anion exchange chromatography and FT-IR spectroscopy were used to analyze the structural characteristics of the polysaccharide.Results showed that the molecular weight of the polysaccharide was 61.6 kD,displaying a single chromatographic peak,and its structure contained a pyran ring.In addition,the polysaccharide was composed of arabinose,galactose,glucose,xylose,mannose and galacturonic acid,with a molar ratio of 2.5∶31∶36∶1∶15∶4.The findings may be useful for future studies on the structure-activity relationship of polysacchaddes from Paecilomyces hepiali Chen & Dai and for the development of new polysaccharide-based products.
国家科技期刊平台
NSTL
万方数据
维普
