Optimization of Extraction of Polysaccharide from Dendrobium officinale and Its Antioxidant Effect on Drosophila melanogaster
The ultrasonic-assisted extraction of polysaccharide from Dendrobium officinale at a 250 W low power was optimized by response surface methodology based on central combination design.Extraction temperature,solvent-to-solid ratio,and ultrasonic treatment time were considered as independent variables.The optimum extraction conditions were determined as follows:extraction temperature 41.74 ℃2,solvent-to-solid ratio 50.26∶1 (mL/g),and ultrasonic treatment time 28.65 min.Under these conditions,the maximum extraction yield of 25.39％ was obtained,which was increased by 18％ compared with the value obtained using conventional hot water extraction with a simultaneous reduction in extraction time of 75％.Moreover,the DPPH and hydroxyl radical scavenging activities of polysaecharide from D.officinale were not reduced during ultrasonic-assisted extraction.Drosophila melanogaster were fed with different doses of the extracted polysaecharide,and the results showed that the polysaccharide at moderate dose provided the best antioxidant activity as evidenced by a 30％ increase in superoxide dismutase (SOD) and catalase (CAT) activity and a 46％ decrease in malondialdehyde (MDA) content of male D.melanogaster,as well as an increase of 60％ in CAT activity and 6％ in SOD activity and a 20％ decrease in MDA content of female D.melanogaster,extending the lifespan by an average of 20％.
国家科技期刊平台
NSTL
万方数据
维普
