超声辅助解冻对秘鲁鱿鱼胴体蛋白氧化与品质的影响
Effect of Ultrasound-Assisted Thawing on Protein Oxidation and Quality of Frozen Jumbo Squid (Dosidicus gigas)
朱文慧 1宦海珍 1李月 1步营 1李学鹏 1励建荣1
作者信息
- 1. 渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州 121013
- 折叠
摘要
本研究旨在探讨超声辅助解冻过程中蛋白质氧化、肌肉结构变化与秘鲁鱿鱼胴体品质的关系.采用不同梯度的超声功率(100、300、500 W),在不同温度(5、10℃)下对秘鲁鱿鱼胴体进行超声辅助解冻处理.经U5-5(5℃、500 W)超声解冻处理的鱿鱼解冻和蒸煮质量损失最少,低场核磁共振实验结果也显示,U5-5超声解冻处理可以降低汁液流失.7种不同解冻方式的鱿鱼羰基含量、总巯基含量、二聚酪氨酸含量和表面疏水性差异显著(P＜0.05).U5-3(5℃、300W)、U5-5超声解冻处理对肌原纤维蛋白羰基含量影响小,U5-3超声解冻组的总巯基含量最高,二聚酪氨酸含量最低,冷藏解冻和U5-1(5℃、100W)超声解冻组蛋白质表面疏水基团暴露最少.拉曼光谱分析结果表明,超声辅助解冻可以延缓α-螺旋向β-折叠、β-转角和无规卷曲结构转化.综上,5℃超声辅助解冻(U5-3、U5-5)可以改善秘鲁鱿鱼产品品质,延缓蛋白氧化.
Abstract
The objective of the present study was to explore the relationship of protein oxidation,muscle structural changes and jumbo squid mantle quality during ultrasound-assisted thawing.Frozen jumbo squid mantles were thawed by ultrasonic treatment using different power gradients (100,300,and 500 W) at different temperatures (5 and 10 ℃).The U5-5 treatment (5 ℃,500 W)caused the smllest thawing and cooking loss.Low-field nuclear magnetic resonance (LF-NMR) suggested that U5-5 could reduce juice loss.The seven different thawing methods showed significant differences in carbonyl content,total sulfhydiyl content,dityrosine content and surface hydrophobicity (P ＜ 0.05).U5-3 (5 ℃,300 W) and U5-5 had little effect on the carbonyl content of myofibrillar protein.U5-3 caused the highest total sulthydryl content and the smallest dityrosine content,while refrigerator thawing and U5-1 (5 ℃,100 W) caused the least exposure of hydrophobic groups on the protein surface.Raman spectroscopy showed that ultrasonic-assisted thawing could retard the conversion of α-helices to β-sheets,β-turns and random coils.These results demonstrate that ultrasonic-assisted thawing at 5 ℃ (U5-3 and U5-5) can improve product quality and defer protein oxidation.
关键词
超声辅助解冻/秘鲁鱿鱼/蛋白氧化/品质/结构变化Key words
ultrasonic-assisted thawing/jumbo squid/protein oxidation/quality/structural transformation引用本文复制引用
基金项目
国家自然科学基金青年科学基金(31901763)
辽宁省“兴辽英才计划”项目(XLYC1807133)
出版年
2021
国家科技期刊平台
NSTL
维普
万方数据