植物乳杆菌XCT 1-10对灰葡萄孢体内和体外生长的影响及其可能机制
In Vitro and in Vivo Effect of Lactobacillus plantarum XCT 1-10 on the Growth of Botrytis cinerea and Its Possible Mechanisms
李灿婴 1程园 1侯佳宝 1葛永红 1杜宏 1白凤翎1
作者信息
- 1. 渤海大学食品科学与工程学院,生鲜农产品贮藏加工及安全控制技术国家地方联合工程研究中心,辽宁锦州 121013
- 折叠
摘要
研究不同浓度(o、102、104、106、108、1010 CFU/mL)植物乳杆菌(Lactobacillus plantarum)XCT 1-10对灰葡萄孢(Botrytis cinerea)菌落生长、孢子萌发以及接种苹果腐烂的影响.结果 表明,106 CFU/mI的L.plantarum XCT 1-10显著抑制了接种B.cinerea的苹果果实病斑扩展,显著抑制了B.cinerea孢子萌发和菌落生长.体外实验表明,L.plantarum XCT 1-10处理提高了 B.cinerea培养液中丙二醛、可溶性糖、可溶性蛋白含量和核酸释放,但降低了相对电导率.结果 表明,L.plantarum XCT 1-10抑制B.cinerea生长的机制可能与破坏其细胞膜有关.
Abstract
The effects of Lactobacillus plantarum XCT 1-10 at different concentrations (0,102,104,106,108,and 1010 CFU/mL) on the colony growth and spore germination of Botrytis cinerea and the decay of apples inoculated with B.cinerea were investigated in this study.The results demonstrated that L.plantarum XCT 1-10 at 106CFU/mL significantly restrained lesion development in apple fruit inoculated with B.cinerea,and distinctly depressed the spore germination and colony growth of B.cinerea.The in vitro test demonstrated that L.plantarum XCT1-10 treatment increased the contents of malondialdehyde (MDA),soluble sugar and soluble proteins,and nucleic acid release,but decreased the relative conductivity of B.cinerea.These findings suggest that L.plantarum XCT 1-10 can suppress the growth of B.cinerea possibly by destroying the cell membrane.
关键词
灰葡萄孢/细胞膜/乳酸菌/菌丝生长Key words
Botrytis cinerea/plasma membrane/lactic acid bacteria/mycelial growth引用本文复制引用
基金项目
国家自然科学基金青年科学基金(31801595)
辽宁省食品科学与工程一流学科专项(LNSPXKBD2020305)
出版年
2021
国家科技期刊平台
NSTL
维普
万方数据