Structural Identification,in Vitro Immunomodulatory and Antitumor Activities of Polysaccharides AHL-P from Arachis hypogaea Leaves
In this study,a polysaccharide(AHL-P)was obtained from Arachis hypogaea leaves by hot water extraction.High performance gel permeation chromatography(HPGPC),Fourier transform infrared spectroscopy(FTIR),nuclear magnetic resonance spectroscopy(NMR),and gas chromatography mass spectrometry(GC-MS)were used to identify the structure of AHL-P.The effects of AHL-P on the proliferation of MFC cells,S180 cells,B cells(Raji),T cells(Jurkat)and RAW 264.7 cells in vitro were also tested by cell counting kit-8(CCK-8).The results indicated that the molecular mass of AHL-P was 12.84 kDa and its monosaccharide composition consisted of glucose,xylose,galactose and arabinose at a ratio of 7∶2∶1∶1.AHL-P was a novel polysaccharide having a backbone chain composed of(1→4,6)-Glc,(1→4)-Xyl and(1→4)-Glc,with(1→4)-Ara and(1→4)-Gal as branches and→1)-Glc as a terminal monosaccharide of the repeating unit.The antitumor activity assay in vitro indicated that the highest inhibitory rate of AHL-P on S180 and MFC cells of 39.07％and 52.22％was observed at a concentration of 5 and 2.5 μg/mL,respectively.The immunomodulatory activity assay in vitro indicated that at 10 μg/mL AHL-P,the proliferation rate of B cells(Raji)and RAW 264.7 cells was 104.39％and 32.25％,respectively.However,AHL-P had no significantly proliferative effect on T cells(Jurkat).This study could provide a scientific basis for the development and utilization of Arachis hypogaea leaves.
国家科技期刊平台
NSTL
万方数据
维普
