Advances in Strain Improvement for the Production of ε-Poly-L-lysine
ε-Poly-L-lysine(ε-PL)is a novel biopolymer consisting of 25-35 L-lysine residues,which is formed by the dehydration condensation of ε-NH2 and a-COOH.ε-PL possesses many excellent characteristics,such as antimicrobial activity,edibility,water solubility,biodegradability,thermostability and nontoxicity.As a natural and safe food preservative,ε-PL possesses many excellent advantages such as thermal stability,edibility,water.solubility,degradability,and broad-spectrum antibacterial activity and has been successfully utilized in Japan,South Korea,the United States,China and other countries.ε-PL is usually produced by fermentation with Streptomyces albulus,and improving ε-PL-producing stains is crucial for enhancing ε-PL production and reducing costs.At present,researchers have obtained microbial strains capable of producing high levels of ε-PL by using physicochemical mutagenesis,ribosome engineering,genome shuffling,genetic engineering and other methods.This review introduces the mechanism of ε-PL biosynthesis and recent progress in strain improvement for the production of ε-PL,and gives an overview of the fermentation process of ε-PL.Finally,this review concludes with an outlook on future research directions.We hope that this review can help promote strain improvement for green biological manufacturing of ε-poly-L-lysine.
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