Prebiotic Properties and High-Density Culture of Bifidobacterium longum B2-01,a Strain Isolated from the Intestinal Tract of Infants
In this study,a Lactobacillus strain was isolated from the feces of healthy 3-month-old infants in Guangzhou,identified and named as Bifidobacterium longum B2-01 by molecular biology,and its probiotic properties,such as in vitro antioxidant activity and hepatoprotective activity,and high-density culture were investigated.The results showed that B2-01 had a strong ability to scavenge 1,1-diphenyl-2-picrylhydrazyl(DPPH),hydroxyl,superoxide anion,and 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonic acid)(ABTS)cation radicals and to resist lipid peroxidation.At the highest concentration of 10％(V/V)in the fermentation supernatant of DMEM medium,strain B2-01 increased the survival rates of L02 cells against hydrogen peroxide damage and alcohol damage to 89.10％and 91.38％,respectively,while significantly reducing the transaminase activity in hepatocytes.The optimal conditions for high-density culture were determined as follows:yeast extract concentration 3.45％,glucose concentration 2.48％,lactose concentration 2.79％,bacteriological peptone concentration 3％,and oligogalactose concentration 2.5％.Under these conditions,the maximum viable bacterial count of 4.20 × 109 CFU/mL was observed in the fermentation broth,which was 6.45 times as high as that before optimization(7.71 x 108 CFU/mL).
Bifidobacterium longuminfant intestineantioxidant activityprotection against alcoholic liverhigh-density culture
万方数据
维普
