Effect of Supplementation with Bacillus subtilis in the Diet of Pigs on Protein and Lipid Oxidation of Pork Stored in High-oxygen Modified Atmosphere Packaging
To investigate the effect of dietary addition of Bacillus subtilis on the antioxidant capacity,meat color,protein oxidation,lipid oxidation and sensory quality of pork during storage in high-oxygen modified atmosphere packaging,12 Duroc × Landrace × Yorkshire pigs were equally allocated into 2 groups that were fed a basal diet alone and in combination with B.subtilis,respectively.Following slaughter,the longissimus dorsi muscle was taken and cut into two parts:one part was used to measure the activity of antioxidant enzymes;the other part was packaged under high oxygen conditions and stored at 4 ℃.The meat color and the contents of malondialdehyde(MDA),carbonyl,and sulfhydryl were measured after 0,5,10,15,and 20 d,and sensory evaluation was performed.The results demonstrated that the activities of superoxide dismutase(SOD),glutathione peroxidase(GSH-Px),and catalase(CAT)significantly increased in the B.subtilis supplementation group compared with those in the control group(P<0.05).Furthermore,dietary supplementation with B.subtilis significantly improved the color of pork stored in high-oxygen modified atmosphere packaging(P<0.05).During low-temperature storage,dietary supplementation with B.subtilis decreased the MDA content(P<0.05)and inhibited the lipid oxidation of pork stored in high-oxygen modified packaging.In addition,it significantly inhibited the formation of carbonyl compounds and the loss of sulfhydryl compounds(P<0.05),and significantly increased the sensory color score of high-oxygen modified atmosphere packaged pork and the overall preference score after opening the package(P<0.05).In summary,dietary addition of B.subtilis could improve the antioxidant capacity of pork,thereby maintaining the color and inhibiting protein and lipid oxidation in pork stored in high-oxygen modified atmosphere packaging,ultimately enhancing the eating quality of pork.