首页|基于二氧化铈/羟基氧化铁纳米酶的比色分析法快速检测泡椒凤爪中过氧化氢

基于二氧化铈/羟基氧化铁纳米酶的比色分析法快速检测泡椒凤爪中过氧化氢

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采用水热法合成二氧化铈(CeO2)修饰的羟基氧化铁(FeOOH),得到纳米材料CeO2/FeOOH。CeO2/FeOOH具有类过氧化物酶活性,可以催化过氧化氢(H2O2)分解产生羟自由基(·OH)和超氧阴离子自由基(O2-·),将3,3',5,5'-四甲基联苯胺(3,3',5,5'-tetramethylbenzidine,TMB)氧化为蓝色的氧化TMB(oxidized TMB,ox-TMB)。基于此,构建基于CeO2/FeOOH纳米酶的H2O2比色传感器。结果表明:该比色传感器对H2O2显示出高灵敏度响应,检测限为3。92 μmol/L,检测范围为10~1 000 μmol/L。此外,该传感器可用于泡椒凤爪中H2O2的快速检测,其中,H2O2加标回收率为90。59%~93。42%,相对标准偏差≤4。25%,具有灵敏、准确、快速和简便等优势。
Colorimetric Method Based on Cerium Dioxide/Iron Oxyhydroxide Nanozyme for Rapid Detection of Hydrogen Peroxide in Chicken Feet with Pickled Pepper
In this study,an innovative cerium dioxide-modified iron oxyhydroxide(CeO2/FeOOH)nanozyme with peroxidase-like activity was synthesized by one-step hydrothermal method.The CeO2/FeOOH nanozyme could catalyze the decomposition of hydrogen peroxide(H2O2)to produce hydroxyl and superoxide anion radicals,which in turn could oxidize 3,3',5,5'-tetramethylbenzidine(TMB)to produce a blue substance(oxidized TMB,ox-TMB).Based on this,a CeO2/FeOOH-based colorimetric sensor was constructed for the detection of H2O2.The results showed that the colorimetric sensor exhibited a highly sensitive response to H2O2 with a detection limit of 3.92 μmol/L and a linear range of 10 to 1 000 μmol/L.The recoveries for spiked samples ranged from 90.59%to 93.42%with relative standard deviations(RSDs)not more than 4.25%.The developed method had the advantages of high sensitivity,high accuracy,speediness and simplicity.

pickled pepper chicken feethydrogen peroxiderapid detectioncolorimetric methodnanozyme

岳晓月、闫曙亮、符龙、赵电波

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郑州轻工业大学食品与生物工程学院,河南郑州 450001

冷链食品加工与安全控制教育部重点实验室,河南郑州 450001

河南省冷链食品质量安全控制重点实验室,河南郑州 450001

泡椒凤爪 过氧化氢 快速检测 比色法 纳米酶

2025

食品科学
北京食品科学研究院

食品科学

北大核心
影响因子:1.327
ISSN:1002-6630
年,卷(期):2025.46(1)