Exploring the Hepatoprotective Effect of Agaricus bisporus Based on Network Pharmacology
To investigate the hepatoprotective components and mechanism of Agaricus bisporus ethanol extract(ABEE),ultra-high performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS)was used to analyze and identify the components of ABEE.The active components and mechanism were analyzed using network pharmacology and a mouse model of CCl4-induced acute liver injury.UPLC-MS/MS analysis identified 75 compounds,of which 15 were predicted by network pharmacology to be related to liver injury,with 11 different targets.Animal experiments showed that ABEE significantly alleviated CCl4-induced acute liver injury in mice.Compared with the model group,the high-dose group(1 000 mg/kg)showed significant recovery in liver function indicators such as aspartate aminotransferase(AST),alanine aminotransferase(ALT),and alkaline phosphatase(AKP)(P<0.001).The antioxidant capacity in mice was significantly enhanced,as evidenced by a significant increase in superoxide dismutase(SOD)and glutathione peroxidase(GSH-Px)activities(P<0.001)and a significant decrease in malondialdehyde(MDA)content(P<0.01).Detection of the mRNA and protein expression levels of relevant genes was performed using real-time quantitative polymerase chain reaction(PCR)and Western blot techniques,showing that compared with the model group,the expression of the Bcl-2 in the high-dose group significantly increased(P<0.001),while the expression of MYC,NF-κB1,RELA and MMP9 significantly decreased(P<0.01).These results confirmed the predictions,indicating that ABEE might exert its hepatoprotective effect by regulating the expression of Bcl-2,MYC,NF-κB1 and RELA through salicylic acid,palmitic acid,linolenic acid,and chrysin,as well as their associated signaling pathways such as chemical carcinogenesis-receptor activation.This study suggests that ABEE may provide liver protection by inhibiting apoptosis,carcinogenesis,and lipid peroxidation caused by free radicals through multiple components,targets,and pathways.
Agaricus bisporushepatoprotectionnetwork pharmacologyultra-high performance liquid chromatography-tandem mass spectrometrymechanism of action
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