Simultaneous Determination of 39 Plant Toxins in Honey by Ultra-high Performance Liquid Chromatography-Tandem Mass Spectrometry with Solid Phase Extraction
To effectively assess food safety risks associated with potential plant toxin residues present in honey,this study aimed to establish and validate a method for the detection of 39 plant toxins in honey using solid-phase extraction combined with ultra-high performance liquid chromatography-tandem mass spectrometry(SPE-UPLC-MS/MS).The developed method involved sample extraction with 0.1％formic acid,purification using a hydrophilic-lipophilic balanced(HLB)cartridge,detection in the multiple reaction monitoring(MRM)mode,and quantification using the matrix-matched external standard method.The calibration curves exhibited good linearity for all the analytes.Average recoveries ranged from 78.7％to 112.8％with relative standard deviation(RSD)between 1.6％and 15.2％(n=6).This method was characterized by ease of operation,high throughput and high sensitivity,and could be used for the monitoring these toxins in honey.Varying levels of pyrrolizidine alkaloids(PAs)and rhodojaponin Ⅲ were detected in some of the 36 tested batches of honey,confirming the presence of phytotoxin residue risks in honey.
solid-phase extractionultra-high performance liquid chromatography-tandem mass spectrometryhoneyplant toxins
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