Protective Effect of Nanoparticles Loaded with Black Rice Anthocyanins on Caco-2 Cell Damage
In this study,bovine serum albumin(BSA)and genipin(GP)were used to prepare BSA nanoparticles loaded with black rice anthocyanins(BRA-BSAGP)to improve the stability and bioactivity of BRA.The interaction between BSAGP and BRA was analyzed,the release characteristics of BRA-BSAGP during simulated gastrointestinal digestion were explored,and the ameliorative effect and mechanism of BRA-BSAGP on Caco-2 cell damage induced by dextran sulfate sodium(DSS)were investigated.BRA-BSAGP showed a uniform spherical morphology with an encapsulation efficiency of(81.10±0.08)%.Fourier transform infrared spectroscopy(FTIR)and X-ray diffraction(XRD)analysis confirmed the successful encapsulation of BRA in BSA nanoparticles.In vitro gastrointestinal digestion demonstrated that BRA-BSAGP nanoparticles effectively delayed the release of BRA,with release percentages of 22.51%and 20.10%under simulated fasting and fed conditions,respectively.BRA-BSAGP significantly increased the survival rate of Caco-2 cells induced by DSS and up-regulated the expression of tight junction proteins(Claudin-1,Occludin,and ZO-1).This study provides a theoretical basis for the application of BRA in developing functional foods for alleviating ulcerative colitis.
black rice anthocyaninsbovine serum albumingenipinCaco-2 cell
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维普
