Determination of Five Fluoroquinolones in Aquatic Products by High Performance Liquid Chromatography with Solid Phase Extraction Using Metal-Organic Framework as Sorbent
A novel metal-organic framework(MOF)material,UiO-66-NH2,was synthesized using a solvothermal method.Subsequently,a high performance liquid chromatography(HPLC)method with solid phase extraction using UiO-66-NH2 was established for the simultaneous detection of five fluoroquinolone(FQ)residues in aquatic products.The sample was extracted with acetonitrile in 1.0%formic acid,degreased with n-hexane,and concentrated by rotary evaporation.The extract was then redissolved in aqueous ammonia solution at pH 8.0.The FQs were enriched and purified with a solid phase extraction(SPE)column filled with 30 mg of UiO-66-NH2 using 6 mL of 20%acetic acid-methanol solution as the eluent.The collected eluate was blown to dryness under nitrogen,redissolved and detected by HPLC with a fluorescence detector.Under the established experimental conditions,good linear relationships were achieved for the five FQs in the concentration range of 0.005-0.50 mg/L,with determination coefficients(R2)of 0.999 9-1.0.The recovery rates ranged from 81.4%to 104.8%with relative standard deviation(RSD)of 1.29%-4.93%,and the limits of detection(LOD)were in the range of 0.21-2.05 μg/kg.The prepared SPE column could be reused up to five times,which was environmentally friendly and cost-effective.The established method had good accuracy,precision,reproducibility,and selectivity,and was suitable for the simultaneous detection of various trace FQ residues in aquatic products.
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