T The fermented rapeseed meal was produced by mixed strains (Laobacillus plantarum R-02 and Bacillus subtilis KGC30141 ) through solid state anaerobic fermentation. The effects of the inoculation amount of two strains,the adding proportion of corn-steep liquor and glucose,and environmental temperature on the content of glucosinolates and crude protein,the loss of materials in fermented rapeseed meal were researched and optimized by a L_(18) (3~5) orthogonal experiment. Results show the optimization conditions as follows: the ratio of inoculation quantity of Laobacillus plantarum and Bacillus subtilis was 6% : 15% ,corn-steep liquor 20%,glucose 3% ,environmental temperature 28℃ ,material to water ratio 1: 1,with the 15-day anaerobic fermentation,the glucosinolates content in the fermented rapeseed meal decreased from 84 μmoL/g( d. b. ) to 33 μmol/g( d. b. ),and the crude protein increased by 4.15% compared to original,the loss of materials was 3.73%.
rapeseed mealanaerobic fermentationglucosinolatesand crude protein