Optimization of γ-PGA Fermentation Medium of Bacillus amyloliquefaciens by Response Surface Methodology
Response surface experiment was designed to identify optimum medium.The optimum medium for Ba cillus amyloliquefaciens L536 was composed of:NaCl 15 g/L,soybean meal 53 g/L,sodium citrate 16 g/L,sodium hydrogen glutamate 40 g/L,NH_4Cl 0.46 g/L,K_2HPO_4 0.625 g/L,MgSO_4·7H_2O 1.25 g/L,MnSO_4·H_2O 0.35 g/ L,CaCl_2·2H_2O 0.2 g/L.Under these conditions,the yield of γ-PGA was 25.81 g/L,increased for about 1.5 times more than before.In order to obtain the optimum fermentation medium for γ-PGA production,the method of re sponse surface experimental design,experimental procedure and results are discussed in this paper.
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