HPLC fingerprint of Pericarpium Zanthoxyli were established by fingerprinting technology. Platisil ODS C18 Column（250 mm x 4.6 mm,5 txm）was used with acetonitrile -water（50： 50） as mobile phase at a flow rate of 0.8 mL/min. The detection wavelength was at 254 nm. The column temperature was at 22 %. The character- istic of Pericarpium Zanthoxyli fingerprint was established by（（ Evaluation Systems of similarity of the chromatographic fingerprint of traditional Chinese medicine, version A~ （researchful version）. Fingerprint of Zanthoxylum Bungeanum Maxim has eleven common peaks, fingerprint of Zanthoxylum Schinifolium Sieb. et Zucc has ten common peaks. Re- ferring to common peaks, similarity value of Zanthoxylum Bungeanum Maxim and Zanthoxylum Schinifolium Sieb. et Zucc was calculated. The results showed fingerprint similarity of Zanthoxylum Bungeanum Maxim was high （amount similarity 〉 0.97 ）, but fingerprint of Zanthoxylum Schinifolium Sieb. et Zucc was low similarity （0.52 - 0.67 ）. Quality of Zanthoxylum Bungeanum Maxim from different origins was more consistent, while Zanthoxylum Schinifolium Sieb. et Zucc has big difference. Characteristic difference between peak of HPLC fingerprint of Zanthoxylum Bungea- num Maxim and Zanthoxylum. Schinifolium Sieb. et Zucc was identified by TOF/MS, and was inferred to hydroxyl-y- sanshool or 2-hvdroxvl-N-isobutvl-2.4.8.10.12-tetradeDentaenoateamide or their structural isomer.
Zanthoxylum Bungeanum MaximZanthoxylum Schinifolium Sieb. et ZuccHPLCfingerprint
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