首页|芝麻香型白酒高温大曲产脂肪酶菌株的筛选与鉴定

芝麻香型白酒高温大曲产脂肪酶菌株的筛选与鉴定

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芝麻香型白酒是我国传统白酒的创新香型,以独特的优雅芝麻香味而闻名,主体香微量成分是在醇、醛、酸、酯多种框架成分含量极其比例关系达到一定基础上再辅以一定量的吡嗪类、呋喃类、酚类等杂环化合物及含硫化合物而形成的复合香,其中产脂肪酶微生物是形成特殊风味物质的关键.研究采用纯培养技术,对山东扳倒井高温大曲不同发酵阶段的大曲样品进行产脂肪酶的微生物菌种的筛选与鉴定.结果表明,采用改进的罗丹明平板法筛选得到15株产脂肪酶菌株,通过活力测定得到6株产脂肪酶活性较高的菌株,对其进行了形态学和分子生物学的鉴定.该研究为今后产脂肪酶功能基因的筛选提供了出发菌株,为芝麻香型白酒香气成分的解析奠定了理论基础.
Screening and identification of lipase-producing strains from high-temperature Daqu in sesame flavor liquor
Sesame flavor liquor is the innovation types of traditional liquor in China,and famous both at home and abroad for unique elegant sesame flavor.The main aroma components are complex flavors of alcohol,aldehyde,acid,ester combined with a certain amount of pyrazines,furans,phenols and heterocyclic compounds.The lipaseproducing microorganisms are key to the formation of special flavor.In this study,the microbial strains producing lipase were screened and identified from different fermentation stages of Shandong bandongjing high-temperature daqu using pure culture technology.The results showed that 15 strains producing lipase were obtained with improved rhodamine platemethods.6 strains with high lipase activities were identified from morphology and molecular characteristics after enzyme activities assays.This study provides original strains for functionallipase-producing genes screening and lays a theoretical foundation for aroma components analysis of sesame flavor liquor.

sesame flavor liquorhigh-temperature daqulipasefunctional strains

翟磊、信春晖、许玲、程宵宵、苏姣姣、姚粟、程池

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中国食品发酵工业研究院,中国工业微生物菌种保藏管理中心,北京,100015

山东扳倒井股份有限公司,山东高青,256300

芝麻香型白酒 高温大曲 脂肪酶 功能性菌株

国家微生物资源平台专项中国食品发酵工业研究院科技发展基金(博士基金)项目

NIMR2015-42015KJFZ-BS-04

2015

食品与发酵工业
中国食品发酵工业研究院 全国食品与发酵工业信息中心

食品与发酵工业

CSTPCDCSCD北大核心
影响因子:0.761
ISSN:0253-990X
年,卷(期):2015.41(12)
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