Screening and identification of lipase-producing strains from high-temperature Daqu in sesame flavor liquor
Sesame flavor liquor is the innovation types of traditional liquor in China,and famous both at home and abroad for unique elegant sesame flavor.The main aroma components are complex flavors of alcohol,aldehyde,acid,ester combined with a certain amount of pyrazines,furans,phenols and heterocyclic compounds.The lipaseproducing microorganisms are key to the formation of special flavor.In this study,the microbial strains producing lipase were screened and identified from different fermentation stages of Shandong bandongjing high-temperature daqu using pure culture technology.The results showed that 15 strains producing lipase were obtained with improved rhodamine platemethods.6 strains with high lipase activities were identified from morphology and molecular characteristics after enzyme activities assays.This study provides original strains for functionallipase-producing genes screening and lays a theoretical foundation for aroma components analysis of sesame flavor liquor.