首页|酵素食品微生物指标与主要功效酶及有机酸分析

酵素食品微生物指标与主要功效酶及有机酸分析

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为了解不同原料自然发酵酵素食品中微生物组成,评价市售酵素食品的理化指标、微生物指标、主要功效酶活力和有机酸组成,对23份发酵期酵素样品进行酵母菌数、乳酸菌数和醋酸菌数测定,对4种市售酵素食品进行总酸、可溶性固形物含量、微生物指标及蛋白酶、脂肪酶、α-淀粉酶、超氧化物歧化酶活力测定,并采用高效液相色谱法对其有机酸的种类及含量进行分析.结果表明,发酵期酵素中广泛存在酵母菌、乳酸菌和醋酸菌;市售酵素食品的功效酶活力偏低,主要有机酸为草酸、苹果酸、乳酸和乙酸.结论:市售酵素食品质量参差不齐,功效酶活力较低,与其宣传功效不符.该研究为了解酵素食品品质、正确看待酵素食品提供了数据参考.
Analysis on microorganism index, main enzymes and organic acids of leaven food
In order to understand the microorganism compositions of natural fermentation leaven food with different raw materials,and evaluate the physical-chemical index,microorganism index,main enzyme activity and organic acids components,the amount of yeast,lactic acid bacteria and acetic acid bacteria was detected.In this paper,the content of total acid and soluble solid,and the microorganism index of commercially available leaven food were detected.At the same time,the activity of protease,lipase,α-amylase and superoxide dismutase was determined,the organic acids was analyzed with high performance liquid chromatography.Results showed that there were yeast,lactic acid bacteria and acetic acid bacteria in leaven in the fermentation period.The activity of main enzymes of the commercially available leaven food was low.The main organic acids of commercially available leaven food were oxalic acid,malic acid,lactic acid and acetic acid.In conclusion,the quality of commercially available leaven food was not uniform.And the low enzyme activity was incompatible with the propaganda.This work could provide data reference for obtaining a correct view of leaven food and comprehending the leaven food quality.

leaven foodmicroorganismenzyme activityorganic acids

张梦梅、刘芳、胡凯弟、胡露、刘金霞、张美佳、刘书亮

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四川农业大学食品学院,四川雅安,625014

四川农业大学食品加工与安全研究所,四川雅安,625014

酵素食品 微生物 酶活 有机酸

四川省农业科技成果转化资金项目

14NZ0012

2017

食品与发酵工业
中国食品发酵工业研究院 全国食品与发酵工业信息中心

食品与发酵工业

CSTPCDCSCD北大核心
影响因子:0.761
ISSN:0253-990X
年,卷(期):2017.43(9)
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