Effects of different concentrations of Cyclosporin A on the quality and apoptosis of beef during postmortem aging
In order to study the effects of Cyclosporine A (CsA) on the quality and apoptosis during Longissimus dorsi muscle postmortem aging,four different concentrations at 0.10,0.20,0.25 and 0.30 mol/L were studied.The changes of pH value,meat color,water holding,tenderness,Cytochrome C (Cyt-c) content and caspase-9,3 activity in the two groups were determined after slaughtering of 12,24,48 and 96 h,respectively.The results showed that L*,and a * in the treatment group were higher than those of the control group within 72 h during postmortem aging;and the change of b * was the opposite.The pH value,the cooking loss and the shear force in the treatment group were higher than those of the control group within 48 h.Meanwhile,the MFI of the treatment group were lower than those in the control group.The content of Cyt-c in the treatment group was higher than that in the control group within 48 h.The activity of caspase-9 in the treatment group was lower than that in the control group within 24 h,and at 48h the change towards the opposite way.With the increase of the treatment solution concentration,the activity of caspase-3 in the two groups increased first and then decreased,and the activity of caspase-3 in the treatment group was higher than that in the control group at 48 h.The above studies demonstrated that CsA treatment has a great impact on the quality of muscle during postmortem aging,and the quality of the changes were concentration-dependent changes in the trend,while CsA can inhibit the occurrence of post-mortem cell apoptosis and thus may affect muscle quality.
Cyclosporin A (CsA)beefquality changeapoptosisCytochrome C (Cyt-c)